Comparative studies of intracellular transport of secretory proteins.

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Comparative studies of intracellular transport of secretory proteins

The physiology of protein intracellular transport and secretion by cell types thought to be free from short-term control has been compared with that of the pancreatic acinar cell, using pulse-chase protocols to follow biosynthetically-labeled secretory products. Data previously obtained (Tartakoff, A.M., and P. Vassalli. J. Exp. Med. 146:1332-1345) has shown that plasma-cell immunoglobulin (Ig)...

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Intracellular Transport of Secretory Proteins in the Pancreatic Exocrine Cell

Since in the pancreatic exocrine cell synthesis and intracellular transport of secretory proteins can be uncoupled (1), it is possible to examine separately the metabolic requirements of the latter process. To this intent, guinea pig pancreatic slices were pulse labeled with leucine-(3)H for 3 min and incubated post-pulse for 37 min in chase medium containing 5 x 10(-4)M cycloheximide and inhib...

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comparative dna interaction studies of antiviral drug, zidovudine and its complex using different instrumental methods

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Intracellular metal transport proteins

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Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes.

We examined the effect of brefeldin A, an antiviral antibiotic, on protein synthesis, intracellular processing, and secretion in primary culture of rat hepatocytes. The secretion was strongly blocked by the drug at 1 microgram/ml and higher concentrations, while the protein synthesis was maintained fairly well. Pulse-chase experiments with [35S]methionine demonstrated that brefeldin A completel...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1978

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.79.3.694